TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| B. subtilis | ||
| DE1 | Prototrophic revertant of W168 trpC2 | 1 |
| PAL1 | purA′-lucGR integrant | This study |
| PAL3 | purA′-lucGR integrant with a cis-control-site (−174 to −55) deletion | This study |
| PAL4 | purA′-lucGR integrant with a −10 promoter mutation | This study |
| NMW | purA′-lucGR integrant with purR::neo disruption | This study |
| N6H | purR6H Nmr ′yabJ integrant of PAL1 | This study |
| N3A | purR6H/D203A Nmr ′yabJ integrant of PAL1 | This study |
| N4A | purR6H/D204A Nmr ′yabJ integrant of PAL1 | This study |
| NPR | purR+ Nmr ′yabJ integrant of PAL1 | This study |
| E. coli | ||
| DH5α F′ | F′ φ80 dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdr17(rK− mK+) supE44 λ− thi-1 gyrA96 relA1 | 5 |
| XL2 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr) Amy Camr] | Stratagene |
| KE94 | F′ hsdS20 (rB− mB−) recA13 ara-14 proA2 lacY1 galK12 rpsL20 (str) xyl-5 mtl-1 supE44 λ− pcnB80 zad::Tn10; used to maintain low plasmid copy number | 11 |
| Plasmids | ||
| pPAL1 | Integration vector with transcriptional purA′-lucGR fusion | This study |
| pPAL3 | pPAL1 derivative with −174 to −55 cis control site replaced by Nmr gene | This study |
| pMW11 | pT7-7 derivative containing purR::neo disruption | 20 |
| pPAL4 | pPAL1 derivative with −10 promoter mutation | This study |
| pR6H | purR6H derivative of pT7-7 | 19 |
| pR6H3A | purR D203A mutation in pR6H | This study |
| pR6H4A | purR D204A mutation in pR6H | This study |
| pN6H2 | ′purR6H Nmr ′yabJ′ derivative of pGEM-7Zf(+) | This study |
| pN3A2 | ′purR6H/D203A Nmr ′yabJ′ derivative of pGEM-7Zf(+) | This study |
| pN4A2 | ′purR6H/D204A Nmr ′yabJ′ derivative of pGEM-7Zf(+) | This study |
| pNPR1 | ′purR Nmr ′yabJ′ derivative of pGEM-7Zf(+) | This study |