TABLE 2.
Effect of purR disruption or deletion of purA 5′-flanking region on transcription of purA
| B. subtilis strain | Operatorb | purR | Mean
luciferase activity ± SD for culture grown as
indicateda
|
|||
|---|---|---|---|---|---|---|
| No purines | Ade | Guo | Ade + Guo | |||
| PAL1 | + | + | 498 ± 153 | 48 ± 5.0 | 2,278 ± 428 | 34 ± 1.4 |
| NMW | + | − | 5,382 ± 817 | 7,514 ± 1,405 | 5,870 ± 2,114 | 7,611 ± 1,128 |
| PAL3 | Δ | + | 5,525 ± 915 | 8,852 ± 1,328 | 5,667 ± 548 | 8,275 ± 1,190 |
a
Values were obtained from three independent experiments. Ade, adenine; Guo, guanosine.
b
The cis control region is either wild type (+) or deleted (Δ). Bacteria were grown as described in Materials and Methods.