TABLE 3.
Regulation of purA in the PRPP binding mutants
| B. subtilis strain | purR | yabJ | Mean
luciferase activity ± SD for culture grown as
indicateda
|
|||
|---|---|---|---|---|---|---|
| No purines | Ade | Guo | Ade + Guo | |||
| NPR | purR | − | 1,186 ± 98 | 822 ± 134 | 3,415 ± 464 | 1,041 ± 196 |
| N6H | purR6H | − | 1,106 ± 221 | 1,357 ± 145 | 5,334 ± 628 | 2,173 ± 370 |
| N3A | purR6H/D203A | − | 726 ± 148 | 984 ± 211 | 932 ± 154 | 1,063 ± 461 |
| N4A | purR6H/D204A | − | 399 ± 74 | 799 ± 146 | 810 ± 59 | 820 ± 35 |
a
Values were obtained from three independent experiments. Ade, adenine; Guo, guanosine.