Maturation medium for NGN2 neurons

Reagent	Final concentration	Amount
Neurobasal medium	n/a	48.3 mL
B-27	1 ×	1 mL
GlutaMAX	1 ×	0.5 mL
BDNF (10 μg/mL)	10 ng/mL	50 μL
NT-3 (10 μg/mL)	10 ng/mL	50 μL
Laminin (10 μg/mL)	0.2 μg/mL	10 μL
MycoZap	1 ×	100 μL
DOX (3 mg/mL, add directly before use)	3 μg/mL	n/a
AraC (add only from day 6)	2 μM	25 μL
Total	n/a	50 mL

Store at 2°C–8°C for up to 2 weeks. Always add fresh DOX directly before use to the medium.