REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, CF™ 488A antibody produced in donkey (1: 1000)	Sigma-Aldrich	Cat#SAB4600033
Anti-MAP2 polyclonal antibody (guinea pig) (1: 1000)	Synaptic Systems	Cat#188004
Anti-Neurofilament mixture of monoclonal antibodies, SMI312 (mouse) (1:500)	BioLegend	Cat#837904
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 (1: 1000)	Invitrogen	Cat#A-11031
Chemicals, peptides, and recombinant proteins
16% Paraformaldehyde (PFA)	Thermo Fisher Scientific	Cat#28906
B27	Gibco	Cat#17504044
BDNF	MACS Miltenyi	Cat#130-096-811
Bovine Serum Albumin	Sigma-Aldrich	Cat#A9647
Cytosin-1-β-D-arabinofuranosid (AraC)	Sigma-Aldrich	Cat#C1768
DMEM/F-12	Gibco	Cat#31330095
Doxycycline hydrochloride (DOX)	Sigma-Aldrich	Cat#D3072
Geltrex	Gibco	Cat#A1413201
GlutaMAX	Gibco	Cat#35050061
Hoechst 33342	Invitrogen	Cat#H3570
Laminin	Sigma-Aldrich	Cat#L2020
MycoZap	Lonza	Cat#VZA-2012
Neurobasal	Gibco	Cat#21103-049
NT-3	Biozol/PeproTech	Cat#450-03
PBS w/o calcium and magnesium	Gibco	Cat#14190144
ROCK inhibitor Y-27632	Enzy Life Sciences	Cat#ALX-270-333-M005
Sodium Azide	Sigma-Aldrich	Cat#71289
StemPro Accutase	Thermo Fisher Scientific	Cat#A1110501
Triton X-100	Bio-Rad	Cat#161-0407
Deposited data
CellProfiler pipeline neurite outgrowth	This paper	https://doi.org/10.5281/zenodo.6642365
Experimental models: Cell lines
Human: BIHi005-A-24 cell line	Berlin Institute of Health	N/A
Software and algorithms
CellProfiler 4.2.1.	Carpenter et al. (2006)	https://cellprofiler.org/
Other
384 well Cell Culture Microplate, PS, F-Bottom, black TC, μCLEAR	Greiner Bio-One	Cat#781091
CytoSmart Cell Counter	Greiner Bio-One	Cat#6749
Operetta® CLS™	PerkinElmer	Cat#HH16000000