FIGURE 6.

PD reduces oxidative stress injury and apoptosis induced by cisplatin. Representative immunofluorescence labelling in the cochlea for caspase-3 [red, (A)] and iNOS [red, (B)], double stained with DAPI in blue. The three dotted areas are SGNs, OHCs and SV. (A,B) Under normal circumstances, caspase-3 and iNOS were barely expressed in SGNs, OHCs and SV. However, apoptosis and oxidative stress damage occurred in cells in the three regions after cisplatin administration, accompanied by high expression of caspase-3 and iNOS, and marked increase in red fluorescence intensity. PD and DEX before cisplatin administration reduced oxidative stress damage and cell apoptosis in the cochlea, showing that the red fluorescence intensity was lower than that of the CDDP group. Scale bar, 100 μm.