TABLE 4.

Functional tests on perinatal tissues.

Perinatal tissues
PnD	Functional in vitro tests	Outcome	Reference
Dehydrated hAM/chorion (EpiFix®)	(i) Cell proliferation assay	(i) Dehydrated hAM/chorion extracts caused a dose-dependent increase in HDF proliferation. (ii) Dehydrated hAM/chorion tissue allografts promoted migration of hMSC. (iii) Growth factors such as EGF, bFGF and TGF-1 were able to elute from dehydrated hAM/chorion into the saline.	(Koob et al., 2013)
	(ii) Chemotaxis assay
	(iii) ELISA assay
Dehydrated hAM/chorion (EpiFix®)	(i) Cell proliferation assay	(i) Dehydrated hAM/chorion extract promoted HMVEC proliferation. (ii) Dehydrated hAM/chorion tissue recruited migration of HUVEC. (iii) Dehydrated hAM/chorion extract increased endogenous production of over 30 angiogenic factors by HMVEC, including GM-CSF, angiogenin, TGF-β3, and HB-EGF Heparin-binding EGF-like growth factor.	(Koob et al., 2014)
	(ii) Chemotaxis assay
	(iii) ELISA assay
hAM	Cytotoxicity assay	Decellularized hAM enhanced the viability of hUC-MSC seeded onto the epithelial surface of hAM.	(Hashemi et al., 2020)

Abbreviations: bFGF: basic fibroblast growth factor, EGF: epidermal growth factor, GM-CSF: granulocyte macrophage colony-stimulating factor, hAM: human amniotic membrane, HB-EGF: Heparin-binding EGF-like growth factor, hMSC: human mesenchymal stromal cells, HMVEC: human microvascular endothelial cells, hUC-MSC: human umbilical cord mesenchymal stromal cells, TGF-1: transforming growth factor 1, TGF-β3: transforming growth factor β3.