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. 2022 Aug 17;12(8):e945. doi: 10.1002/ctm2.945

FIGURE 3.

FIGURE 3

FAM135B affects ATM activity in DNA repair. (A and B) KYSE450 (A) and KYSE510 (B) cells were transfected with siFAM135B or siCTRL for 48 h and subsequently treated with 10‐μM BLM for 12 h, then cells were harvested at the indicated time point and subjected to western blots with indicated antibodies. (C and D) KYSE30 cells were transfected with FAM135B‐FLAG or empty vector (EV) for 48 h and subsequently treated with 10‐μM BLM for 12 h (C) or 6‐Gy IR (D), then cells were harvested at the indicated time points and subjected to western blot with indicated antibodies.