TABLE 3.
Oxidative damage and sensitivity to H2O2 in exponentially growing E. colia
| Strain (relevant genotype) | Mutation frequency (Rifr
colonies/109 cells)
|
H2O2 sensitivity (killing zone, mm) | |
|---|---|---|---|
| Without poxyS | With poxyS | ||
| AB1157 (oxyRS+) | 10 ± 2 | 6 ± 5 | 8 ± 1 |
| BGF416 (oxyR+ ΔoxyS) | 11 ± 2 | 5 ± 1 | 9 ± 1 |
| BGF611 (oxyRS+ katG) | 16 ± 1 | 8 ± 1 | 10 ± 1 |
| BGF420 (oxyS katG) | 28 ± 2 | 20 ± 2 | 12 ± 1 |
| RK4936 (oxyRS+) | 7 ± 1 | 5 ± 1 | 7 ± 2 |
| TA4112 (ΔoxyRS) | 80 ± 8 | 100 ± 10 | 17 ± 1 |
a
Three-hour cultures were plated on LB plates with rifampin or LB plates with a filter paper soaked in H2O2, as described in the text. Values are means of four to six independent experiments ± SEMs.