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. 2022 Aug 4;13:919290. doi: 10.3389/fpls.2022.919290

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Copyright © 2022 Jedličková, Mácová, Štefková, Butula, Staveníková, Sedláček and Robert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Table summarizing the mutagenesis efficiency of the CRISPR/Cas9 constructs for gRNA loci in BnaA02.TAA1 (guide1 and guide2) and BnaC02.TAA1 (guide1 and guide2). The mutagenesis efficiency is calculated as the percentage of the type of mutation on the total loci per construct (16–30 independent hairy root lines per construct). Data are pulled for a comparison of efficiency. The percentages of the type of alleles per loci (WT, mutated loci) and types of mutations (homozygote, bi-allele, heterozygote, chimera) over the total number of (mutated) loci are counted. Homozygote: the two alleles of a locus have an identical mutation. Bi-allele: the two alleles of a locus have different mutations. Heterozygote: only one allele is mutated. Chimera: more than two mutations per locus. Supporting data are shown in Supplementary Table 4.