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CD44 in GAMs mediates increase in MMP9. (A) 2 × 104 GL261 cells were implanted in 5-8 weeks old floxed or CD44 cKO mice. After 21 days, myeloid cells from tumor-bearing brains were isolated by MACS with Cd11b microbeads. Total RNA was extracted and processed for qRT-PCR analysis. (B) mRNA levels of TNF-α, IL-1b, MMP9 and MT1-MMP. Bar charts represent mRNA levels as fold change to floxed mice. Data are presented as mean ± SD. Student’s t-Test, * p < 0.05.
