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. 2022 Jul 12;298(8):102253. doi: 10.1016/j.jbc.2022.102253

Figure 3.

Figure 3

Comparative expression of six different proteins. Each protein is expressed in parallel using three types of media: LB, 1H-M9, and 2H-M9. IPTG-induced products are marked with red arrows. Samples are collected before harvest centrifugation. A 20-μl aliquot of each culture is mixed with 20 μl 2xSDS sample buffer, denatured at 95 °C for 30 min. Equal amounts of sample (20 μl) are loaded in each lane on SDS-PAGE gel. Relative intensities from digitization of bands for LB, 1H-M9, and 2H M-9 are shown for all six proteins, respectively. aNMT (N-myristoyltransferase) is coexpressed with Arf1 for posttranslational modification.