Skip to main content
. 2022 Jul 12;298(8):102253. doi: 10.1016/j.jbc.2022.102253

TableĀ 1.

Mass spectrometry data and isotopic labeling efficiencies

graphic file with name fx1.gif

a Mass represents the replacement of nonexchangeable protons with 2H. All exchangeable -NH, -NH2, -SH, -OH, and -COOH protons are calculated as 1H, representing back exchange during purification and mass spectrometry analyses. The mass spectrometry analyses were performed on an electrospray LC-MS that ensures full protonation of all exchangeable sites. The calculations were performed with the software package Protein Sequence Analysis, developed by Ira Palmer, NIH.

b Predicted mass computed using the amino acid sequence and the software package Protein Sequence Analysis, developed by Ira Palmer, NIH.