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. 2022 Jul 14;63(8):100249. doi: 10.1016/j.jlr.2022.100249

Fig. 2.

Fig. 2

ARI and TRZ treatment alter sterol biosynthesis in primary neuronal and astrocyte cultures. A: 7-DHC levels, B: DES levels, and C: 7-DHC/CHOL. Cortical neurons and astrocytes were cultured and treated with five different concentrations of ARI, TRZ, or ARI+TRZ for 3–9 days. Data from 6 days of culturing are presented for vehicle treatment, 5 nmol ARI, 20 nmol TRZ, and ARI+TRZ (5 nmol+20 nmol). The response to all concentrations of ARI, TRZ, and ARI+TRZ drug treatments can be found in supplemental Figs. S2–S4. Treatments compared with vehicle exposure that reached significance are marked with black asterisks. Unpaired, two-tailed t test was used for statistical comparison of different treatments; ∗∗∗∗P<0.0001. Abbreviations: ARI, aripiprazole; TRZ, trazodone; ARI+TRZ, combined treatment with aripiprazole and trazodone; CNTR (vehicle), control group treated with vehicle. Note that treatment with ARI+TRZ showed an additive effect, increasing the 7-DHC/CHOL ratio by 15- to 20-fold over vehicle-treated cultures (P<0.001), compared with 8- to 9-fold and 6- to 10-fold baseline change for single drug ARI and TRZ treatment, respectively.