Fig. 6.

ARI and TRZ treatment caused a strong and significant decrease in DES levels across all brain regions of C57BL/6 mice. Each symbol represents a regional brain sample from a single mouse. The x-axis denotes animal groups; y-axis denotes DES concentration normalized to mg of protein. Black asterisk denotes comparison with baseline levels. Two-tailed groupwise t test was used for statistical comparisons, ∗P<0.05, ∗∗P<0.01, ∗∗∗P<0.001, ∗∗∗∗P<0.0001. Abbreviations: ARI, aripiprazole; TRZ, trazodone; ARI+TRZ, combined treatment with aripiprazole and trazodone. Note the strong DES decrease by all treatments. Percentage of desmosterol decrease for each brain region is presented in supplemental Fig. S6B.