Fig. 1.

Whole-cell and Kac peptide proteomics analysis between OXY^Sand OXY^Rstrains.A, the Kac profiles in OXY^S and OXY^R strains by Western blotting. The left figure is the Coomassie blue staining as loading control. B and C, correlation coefficient analysis of whole-protein and Kac peptide proteomics in three biological repeats for each group, respectively. D, the distribution of mass error and the identified score of Kac-modified peptides. E, the frequency distribution of protein/peptide ratio (OXY^Rversus OXY^S) in both whole-cell protein and Kac-modified peptide proteomics. F, the overlapping proteins between altered and total identified proteins in whole-cell proteomics. G, the overlapping Kac proteins/peptides between altered and total identified Kac proteins/peptides in Kac proteomics. H and I, volcano plot of the p-values versus the log2 protein abundance differences between OXY^S and OXY^R, with proteins or peptides outside the significance lines colored in orange or blue, respectively. Some representative protein names and Kac sites are highlighted. OXY^S, oxytetracycline sensitive; OXY^R, oxytetracycline resistance; Kac, lysine acetylation.