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. 2022 Aug 16;219(10):e20220780. doi: 10.1084/jem.20220780

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© 2022 Lim et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 3. — SARS-CoV-2–specific response of nasal and circulating T cells. (A) Paired analysis of IFN-γ SFU frequency in nasal cells (left) and PBMCs (right) stimulated with SARS-CoV-2 peptide pools of vaccinated with breakthrough infection (n = 10) tested 1–2 mo after recovery. Pie charts represent the percentage of IFN-γ SFU reactive to the individual SARS-CoV-2 peptides pools; (B) Representative ELISpot wells from nasal cells and PBMCs. (C) Paired analysis of cytokines (IFN-γ and IL-2) secreted in whole blood (320 μl whole blood and 80 μl medium; unnormalized) and nasal cells (30 μl of medium; normalized to 100,000 lymphocytes/well) after stimulation with different SARS-CoV-2 peptide pools.