Skip to main content
. 2022 Aug 4;13:969499. doi: 10.3389/fmicb.2022.969499

Figure 5.

Figure 5

Overexpression of Bclae1 reduced ABA synthesis in B. cinerea TB-31. (A) The TB-31 and 3 randomly selected Bclae1-OE mutants (Bclae1-OE-X4, Bclae1-OE-X8, Bclae1-OE-X12) were grown on PDA. Samples for the quantitative determination of ABA production were collected at 6–12 days. The error bars indicate the standard errors of the mean for three replicate cultures (n = 3). Asterisks indicate significant differences in ABA production between selected mutants and TB-31 (p < 0.05). (B) RT-qPCR examining the transcriptional levels of ABA gene cluster, BcabaR1 and Bclae1 in Bclae1-OE-X4 and TB-31. The relative transcriptional levels of selected genes were obtained after normalization to the constitutive tubulin reference gene (BC1G_05600) at 6 days. The relative values for selected genes transcription at 6 days in TB-31 were arbitrarily assigned as 100%. Shown are means and SEM, n = 3 independent biological replicates. ***p < 0.001 versus the same genes of the TB-31 group.