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. Author manuscript; available in PMC: 2022 Aug 18.
Published in final edited form as: Chin J Dent Res. 2018;21(2):113–118. doi: 10.3290/j.cjdr.a40437

Figure 4. Jmdj7 knockdown enhances osteoclastogenesis in BMMs.

Figure 4.

(A) Control siRNA (CTLsi) and Jmjd7 siRNA (Jmjd7si) were transfected into the primary BMMs. Transfected BMMs were treated with or without RANKL (100 ng/ml) in the presence of M-CSF (50 ng/ml) for 5 days and stained for TRAP. (B) TRAP positive cells containing more than three or more nuclei were counted as osteoclasts. (C) CTLsi- or Jmjd7si-transfected primary BMMs were treated with or without RANKL (100 ng/ml) in the presence of M-CSF (50 ng/ml) for 3 days. Cells were harvested and subjected to RNA isolation, cDNA synthesis, and qRT-PCR for the expression of Nfac1, Acp5, and Traf6.