KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal CNOT6L antibody | (Morita et al., 2019) | #CNOT6L-B |
| Mouse monoclonal CNOT6L antibody | (Morita et al., 2019) | #694-3 |
| Rabbit monoclonal CNOT1 antibody | Cell Signaling Technology | #44613 |
| Rabbit polyclonal CNOT3 antibody | (Morita et al., 2019) | #CNOT3-full |
| Sheep polyclonal GDF15 antibody | R&D Systems | AF6385 |
| Mouse monoclonal GDF15 antibody | Santa Cruz Biotechnology | sc-377195 |
| Mouse monoclonal FGF21 antibody | Santa Cruz Biotechnology | sc-81946 |
| Mouse monoclonal α-tubulin antibody | Cell Signaling Technology | #2144 |
| Mouse monoclonal β-actin antibody | Cell Signaling Technology | #4970 |
| Mouse monoclonal Myc antibody | Bioshop Canada Inc. | TAG003 |
| Mouse monoclonal Flag M2 antibody | Sigma-Aldrich | F1804 |
| Rabbit polyclonal c-Fos antibody | Sigma-Aldrich | F7799 |
| HRP-linked anti-mouse IgG (light chain specific) | Jackson ImmunoResearch | 115-035-174 |
| HRP-linked anti-rabbit IgG (light chain specific) | Jackson ImmunoResearch | 211-032-171 |
| HRP-linked anti-mouse IgG | Cell Signaling Technology | #7076 |
| HRP-linked anti-rabbit IgG | Cell Signaling Technology | #7074 |
| Anti-rabbit IgG, Alexa Fluor 568 | Thermo Fisher Scientific Inc. | A10042 |
| Bacterial and virus strains | ||
| AAV8-TBG-Cre | Addgene | 107787-AAV8 |
| AAV8-TBG-Cont | Addgene | 105536-AAV8 |
| Escherichia coli strain BL21 Competent Cell | Thermo Fisher Scientific Inc. | EC0114 |
| Adenovirus | Takara Inc. | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| CNOT6L (residues 158–555 a.a.) | (Wang et al., 2010) | N/A |
| CNOT6 (residues 153-557a.a.) | (Wang et al., 2010) | N/A |
| CNOT7 (Full length) | (Yang et al., 2008) | N/A |
| CNOT8 (Full length) | (Yang et al., 2008) | N/A |
| Glutathione-Sepharose 4B | GenScript | L00206 |
| HRV GST-3C protease | Acrobiosystem | 3CCN31331000U |
| BML-2840 ICCB Known Bioactives Library | Enzo Life Sciences, Inc. | BML-2840-0500 |
| BML-2865 Natural Product Library | Enzo Life Sciences, Inc. | BML-2865-0500 |
| Isopropyl β-D-1-thiogalactopyranoside | Thermo Fisher Scientific Inc. | 34060 |
| Dynabeads Protein G | Thermo Fisher Scientific Inc. | 10004D |
| TRIzol Reagent | Thermo Fisher Scientific Inc. | 15596018 |
| GlycoBlue Coprecipitant | Thermo Fisher Scientific Inc. | AM9516 |
| Actinomycin D | Sigma-Aldrich | A1410 |
| DMSO | Corning | 25950CQC |
| WY-14643 | Cayman Chemical | 7073010 |
| Thapsigargin | Sigma-Aldrich | T9033 |
| Tris | Thermo Fisher Scientific Inc. | BP152 |
| EDTA | Thermo Fisher Scientific Inc. | BP118 |
| cOmplete™, EDTA-free Protease Inhibitor Cocktail | Sigma-Aldrich | 11873580001 |
| IGEPAL CA-630 (NP-40) | Sigma-Aldrich | 56741 |
| Triton X-100 | Thermo Fisher Scientific Inc. | BP151 |
| Sodium deoxycholate | Thermo Fisher Scientific Inc. | BP349 |
| HEPES | Thermo Fisher Scientific Inc. | BP310 |
| Cycloheximide | Sigma-Aldrich | C7698 |
| Sucrose | Thermo Fisher Scientific Inc. | BP220 |
| NaCl | Thermo Fisher Scientific Inc. | BP358 |
| KCl | Thermo Fisher Scientific Inc. | BP366 |
| MgCl2 | Thermo Fisher Scientific Inc. | M33 |
| DMEM | Corning | 10-013-CV |
| FBS | Sigma-Aldrich | F8067 |
| Penicillin-Streptomycin Solution | Corning | 30-002-Cl |
| Percoll | Sigma-Aldrich | P7828 |
| Perfusion medium | GIBCO | 17701-038 |
| Liver Digest Medium | GIBCO | 17703-034 |
| William’s E Medium | GIBCO | A1217601 |
| Primary Hepatocyte Thawing and Plating Supplements | GIBCO | CM3000 |
| Primary Hepatocyte Maintenance Supplements | GIBCO | CM4000 |
| Collagen type I | Corning | 354236 |
| Insulin | Novo Nordisk | NDC 0169-1833-02 |
| LY2405319 | (Gaich et al., 2013) | N/A |
| Poly(I:C) | InvivoGen | tlrl-pic |
| High-fat diet | Research Diets, Inc. | D12492 |
| Standard diet | Research Diets, Inc. | D12450B |
| 10% Formalin | Thermo Fisher Scientific Inc. | 245-684 |
| 2-propanol | Thermo Fisher Scientific Inc. | A416 |
| O.C.T. compound | Sakura Finetek USA, Inc. | 4583 |
| VECTASHIELD® Hardset Antifade Mounting Medium with DAPI | Thermo Fisher Scientific Inc. | H-1500 |
| Critical commercial assays | ||
| Amicon Ultra-4 10K centrifuge filter | Millipore | Z648027 |
| COSMOSIL 3C18-MS-II | Nacalai Tesque | 05514-01 |
| Magnetic Separator | Invitrogen | 12321D |
| RNeasy Mini Kit | Qiagen | NC9677589 |
| High-Capacity cDNA Reverse Transcription Kit | Applied Biosystems | 4368814 |
| PowerUp SYBR Green Master Mix | Applied Biosystems | A25742 |
| TruSeq Stranded mRNA Library Prep | Illumina, Inc. | 20020594 |
| Lipofectamine 3000 | Invitrogen | L3000-015 |
| Dual-Glo Luciferase Assay System | Promega | PR-E2940 |
| Bio-Rad protein assay | Bio-Rad Laboratories, Inc. | 5000006 |
| 24GA 0.75IN 0.7x19mm yellow, closed IV catheter | BD Medical | BD 383511 |
| 100μm cell strainer | Falcon | 352360 |
| Glucose meter | Bayer | BD15789 |
| LabAssay Triglyceride | Wako | 290-63701 |
| Autokit Total Ketone Bodies | Wako | 415-73301 411-73401 |
| FGF21 MOUSE ELISA | BioVender Inc. | RD291108200R |
| Mouse/Rat GDF-15 Quantikine ELISA Kit | R&D Systems | MGD150 |
| Ultra Sensitive Mouse Insulin ELISA Kit | Crystal Chem Inc. | 90080 |
| Deposited data | ||
| RIP-CHIP raw data | (Morita et al., 2019) | GEO: GSE62365 |
| RNA-seq raw data from livers | This paper | GEO: GSE185851 |
| Experimental models: Cell lines | ||
| Huh7 cells | JCRB | JCRB0403 |
| Mouse primary hepatocytes | This paper | N/A |
| 293T cells | ATCC | CRL-3216 |
| 293 | ATCC | CRL-1573 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | Jackson laboratory | 000664 |
| Mouse: Cnot6l−/− | (Morita et al., 2019) | N/A |
| Mouse: Fgf21f/f | Jackson laboratory | 022361 |
| Mouse: Alb-cre | Jackson laboratory | 003574 |
| Mouse: Cnot6ltm1a(EUCOMM)Wts | EMMA | N/A |
| Mouse: Cnot6tm1a(KOMP)Wtsi | MMRRC | N/A |
| Mouse: Cnot6lf/f | This paper | N/A |
| Mouse: Cnot6f/f | This paper | N/A |
| Mouse: ACTB-FLPe | Jackson laboratory | 005703 |
| Mouse: Gdf15tm1a(KOMP)Wtsi | MMRRC | N/A |
| Oligonucleotides | ||
| 6-FAM-labeled poly(A) RNA | Sigma-Aldrich | N/A |
| TAMRA-labeled oligo (dT) DNA | Sigma-Aldrich | N/A |
| FITC-labeled poly(A)20 RNA | Sigma-Aldrich | N/A |
| Recombinant DNA | ||
| pGL3-control | Promega | PR-E1741 |
| pGL3-3’UTR of human GDF15 mRNA | This paper | N/A |
| pGL3-3’UTR of mouse Gdf15 mRNA | This paper | N/A |
| pGL3-3’UTR of human FGF21 mRNA | This paper | N/A |
| pGL3-3’UTR of mouse Fgf21 mRNA | This paper | N/A |
| pRL-TK | Promega | PR-E2241 |
| pBAsi-mU6-shGfp | Takara Bio. | 3222 |
| pBAsi-mU6-shGdf15 | This paper | N/A |
| pBAsi-mU6-shFgf21 | This paper | N/A |
| pBAsi-mU6-shCnot6 | This paper | N/A |
| pBAsi-mU6-shCnot6l | This paper | N/A |
| pAxcwit2-shGfp | Takara Bio. | 6170 |
| pAxcwit2-shGdf15 | This paper | N/A |
| pAxcwit2-shFgf21 | This paper | N/A |
| pAxcwit2-shCnot6 | This paper | N/A |
| pAxcwit2-shCnot6l | This paper | N/A |
| pAAV.TBG.PI.Cre.rBG | Addgene | 107787 |
| pAAV.TBG.PI.Null.bGH | Addgene | 105536 |
| pCMV-Myc-CNOT6L | This paper | N/A |
| pcDNA3-flag-YTHDF2 | Addgene | 52300 |
| Software and algorithms | ||
| Molecular Operating Environment | Chemical Computing Group | https://www.chemcomp.com/ |
| GraphPad Prism 7 | GraphPad Software | https://www.graphpad.com/ |
| Endnote | Clarivate | https://endnote.com/ |
| Illustrator 2021 | Adobe | https://www.adobe.com/ |
| Photoshop 2021 | Adobe | https://www.adobe.com/ |
| Other | ||
| iBright FL1000 Imaging System | Thermo Fisher Scientific Inc. | A32752 |
| A Biacore system | GE Healthcare | 28975001 |
| Liquid Chromatograph Mass Spectrometer | Shimadzu | LCMS-8050 |
| NanoDrop Onec | Thermo Fisher Scientific Inc. | 027420P21H2 |
| QuantStudio 3 Real-Time PCR System | Thermo Fisher Scientific Inc. | A28136 |
| Varioskan LUX Multimode Microplate Reader | Thermo Fisher Scientific Inc. | VL0L00D0 |
| 4-lane motorized treadmill | Columbus Instruments | 1012M-4 |
| Fluorescence microscopy | Keyence | BZ-X800 |