Figure 4.

Reaction-time-dependent changes in the citrulline levels at stable PAD3 concentrations detected using western blotting. In the upper figure, an anti-AMC primary antibody was used to detect the autocitrullinated PAD3. In the lower figure, an anti-PAD3 primary antibody was used to detect PAD3 (PAD3 concentration: 0.05 mg/mL; CaCl₂ concentration during the PAD3 autocitrullination reaction: 10 mM). Anti-modified citrulline (AMC); peptidylarginine deiminase 3 (PAD3).