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. 2022 Aug 18;24:199. doi: 10.1186/s13075-022-02850-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — CC-99677 inhibited the production of TNF, IL-6, IL-17A, and MCP-1 from SEB-1/IL-2-stimulated human PBMCs. TNF (a), IL-6 (b), IL-17A (c), and MCP-1 (d) inhibition in SEB/IL-2-stimulated PBMCs from patients with AS and healthy volunteers. PBMCs were treated with CC-99677 for 1 h followed by SEB + IL-2 stimulation for 72 h. Magnetic beads were used to assess TNF, IL-6, IL-17A, and MCP-1. Each sample was analyzed in duplicate, and data were normalized to the stimulation control (100%). The mean percent inhibition is indicated by a horizontal line with error bars for the standard error of the mean; significance was calculated using the one-way ANOVA with Dunnett’s post-test algorithms in GraphPad Prism version 5.01. Gray symbols indicate the data points from healthy donors (n = 10), and blue symbols indicate the data points from donors with AS (n = 30). ANOVA, analysis of variance; AS, ankylosing spondylitis; IL, interleukin; MCP-1, monocyte chemoattractant protein 1; PBMC, peripheral blood mononuclear cell; SEB, Staphylococcal enterotoxin B; stim ctrl, stimulation control; TNF, tumor necrosis factor. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05