Fig. 1.

Pyroptosis in the murine TBI model. To assess the expression of pyroptosis-related proteins, TBI mice were sacrificed and brain tissues were removed at 12 h, 24 h, 48 h and 72 h post-TBI surgery. a Representative protein bands and corresponding grayscale values of NLRP3, pro-Caspase-1, Caspase-1 p20, GSDMD, and cleaved-GSDMD performed by western blotting. b–d Representative double immunofluorescence staining photographs and quantification of Iba-1 (green) with NLRP3 (red), Caspase-1 p20 (red), or GSDMD (red) in the injured cortex. Scale bar = 50 μm. All data are represented as means ± SD (n = 5 mice/group) and compared by t-test. *p < 0.05, **p < 0.01, ***p < 0.001 versus sham group