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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Cancer Lett. 2022 May 16;540:215726. doi: 10.1016/j.canlet.2022.215726

Fig. 4. miR-1290 and miR-1246 inhibition decreases astrocyte activation and mammosphere formation.

Fig. 4.

(A) GFAP expression in astrocytes stimulated by CM from SKBRM cells overexpressing tGLI1 with miR-1290 or miR-1246 inhibitors (SKBRM-tGLI1-miR-Off-1290 or -1246). 10× magnification. (B) Mammosphere assay of SKBRM-tGLI1-miR-Off-Control, −1290, or −1246 cells. 5× magnification. Scale bar indicates 100 μm. (C–D) RT-qPCR of miR-1290 and miR-1246 target genes, respectively, in SKBRM-tGLI1-miROff-1290 (Off-1290) and miR-1246 (Off-1246). One-way ANOVA was used in Panel A–B to compute p-values. Student’s t-test (two-tailed) was used in Panels C–D to derive p-values. N = 3 experimental replicates.