|
Antibodies
|
| mouse anti-Wg (4D4) |
Developmental Studies Hybridoma Bank |
RRID: AB_528512 |
| mouse anti-dMMP1 (14A3D2) |
Developmental Studies Hybridoma Bank |
RRID: AB_579782 |
| mouse anti-dMMP1 (3A6B4) |
Developmental Studies Hybridoma Bank |
RRID: AB_579780 |
| mouse anti-dMMP1 (5H7B11) |
Developmental Studies Hybridoma Bank |
RRID: AB_579779 |
| rat anti-Elav (Elav-7E8A10) |
Developmental Studies Hybridoma Bank |
RRID: AB_528218 |
| mouse anti-beta-galactosidase (40-1a) |
Developmental Studies Hybridoma Bank |
RRID: AB_528100 |
| mouse anti-nubbin (nub 2D4) |
Developmental Studies Hybridoma Bank |
RRID: AB_2722119 |
| rat anti-Zfh2 |
66
|
RRID: AB_2569892 |
| rat anti-Pvf1 |
67
|
RRID: AB_2569381 |
| rabbit anti-cleaved Death Caspase-1 |
Cell Signaling |
RRID: AB_2721060 |
| rabbit anti-Phospho-Histone H3 (PHH3) |
Millipore-Sigma |
Product #: 369A-1 |
| goat anti-rat IgG- Alexa Fluor® 647 |
Cell Signaling |
RRID: AB_1904017 |
| goat anti-mouse IgG- Alexa Fluor® 555 |
Cell Signaling |
RRID: AB_1904022 |
| goat anti-rabbit IgG- Alexa Fluor® 647 |
Cell Signaling |
RRID: AB_10693544 |
| goat anti-chicken IgG- Alexa Fluor® 647 |
Thermo Fisher |
RRID: AB_2535866 |
|
|
|
|
Chemicals, peptides, and recombinant proteins
|
| DAPI |
Sigma Aldrich |
|
| Schneider’s Drosophila Media |
Thermo Fisher |
Cat#21720024 |
|
|
|
|
Critical commercial assays
|
| 10× Genomics v2 Chip + v3.1 Chip |
10× Genomics |
10xgenomics.com
|
| HCR reagents (hairpins and buffers) |
Molecular Instruments |
molecularinstruments.com
|
| HCR probes |
Molecular Instruments |
molecularinstruments.com
|
| EdU Assay: Click-iT EdU Cell Proliferation Kit (C10338 and C10340) |
ThermoFisher Scientific |
thermofisher.com
|
|
|
|
|
Deposited data
|
| Single-cell transcriptomic data: wild-type and Ets21C-mutant regenerating wing discs (two replicates for each genotype) |
This paper |
GSE174326 |
|
|
|
|
Experimental models: Organisms/strains
|
|
rn-GAL4, tub-GAL80ts, UAS-egr
|
7
|
Bl51280 |
|
rn-GAL4, tub-GAL80ts, UAS-rpr
|
7
|
|
|
Ets21CΔ10
|
39
|
|
|
UAS-Ets21C (UAS-Ets21C-long)
|
39
|
|
|
UAS-Ets21C-RNAi
|
39
|
|
|
Ets21C-GFP (Pbac-Ets21C-GFP.FLAGVK00033) |
Bloomington Stock Center |
Bl38639 |
|
Him-GFP
|
62
|
|
|
Ilp8-GFP (Ilp8MI00727) |
Bloomington Stock Center |
Bl33079 |
|
Df(2L)BSC456
|
Bloomington Stock Center |
Bl24960 |
|
Df(2L)BSC107
|
Bloomington Stock Center |
Bl8673 |
|
hh-Gal4
|
P-GAL4 hhGal4
|
FBti0017278 |
|
rn-Gal4
|
P-Gaw rnGAL4-5
|
FBti0023720 |
|
UAS-hepwt
|
P-w[+mC]=UAS-hep.B
|
Bl9308 |
|
UAS-kay-RNAi
|
Bloomington Stock Center |
Bl33379 |
|
UAS-JNKDN (UAS-bsk-K53R)
|
Bloomington Stock Center |
Bl9311 |
|
Ubi-FRT-stop-FRT-GFPnls
|
63
|
Bl32251 |
|
3XUAS-FLP.G5.PEST
|
Bloomington Stock Center |
Bl55809 |
|
lexAOp-FLP
|
Bloomington Stock Center |
Bl55819 |
|
GMR26E03-lexA
|
64
|
Bl54354 |
|
arm-lacZ FRT40A
|
Bloomington Stock Center |
Bl7371 |
|
FRT40A
|
Bloomington Stock Center |
Bl1816 |
|
hsFLP; ubi-RFP, FRT40A
|
Bloomington Stock Center |
Bl34500 |
|
Act5C-FRT-stop-FRT-GAL4
|
Bloomington Stock Center |
Bl4780 |
|
hsFLP
|
Bloomington Stock Center |
Bl8862 |
|
arm-lacZ FRT40A
|
Bloomington Stock Center |
Bl7371 |
|
eyFLP
|
Bloomington Stock Center |
Bl5580 |
| AP-1-activity reporter (AP-1-GFP) |
65
|
Bl59010 |
|
Act5C>FRT.CD2>GAL4, UAS-RFP
|
Bloomington Stock Center |
BL:30558 |
|
scrib1
|
61
|
|
|
scrib2
|
57
|
|
|
UAS-his::RFP
|
60
|
|
|
|
|
|
Software and algorithms
|
| scvi-tools |
21
|
scvi-tools.org
|
| scANVI |
74
|
scvi-tools.org
|
| Seurat |
75
|
satijalab.org/seurat
|
| Python |
73
|
python.org
|
| Scanpy |
76
|
scanpy.readthedocs.io
|
| UMAP |
78
|
umap-learn.readthedocs.io
|
| Image J / Fiji |
68
|
fiji.sc
|
| Slingshot |
79
|
bioconductor.org/packages/release/bioc/html/slingshot.html
|
| 10× Genomics CellRanger |
71
|
10xgenomics.com
|
|
|
|
|
|
|
|
Other
|
|
|
| Single-cell transcriptomics: Developing wing discs (two time points, two replicates) |
19
|
GSE155543 |
| Single-cell transcriptomics: scrib tumorous wing discs (four time points) |
22
|
GSE130566 |
| Code for single-cell analysis (in R and Python) |
This paper |
https://github.com/HariharanLab/Worley_Everetts_Yasutomi
|
