Figure 2: Infection of HAOs with SARS-CoV-2.

Organoids were infected at an MOI of 1 for 2 hours, and then washed and fresh medium added. Organoids were incubated for the length of time indicated (A) Plaque assay comparing wildtype and ACE2 overexpressing organoids at 24 hours post-infection. (B) Plaque assay comparing wildtype and ACE2 overexpressing organoids at 72 hours post-infection. (C) Representative images of plaque assays performed on supernatant from SARS-CoV-2 infection of wildtype and ACE2 overexpressing organoids. (D) Representative images of infected HAOs at 24 and 72 hours stained for dsRNA (green). (E) Quantification of percent of total DAPI+ cells expression dsRNA across three experiments at 24 and 72 hours.