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[Preprint]. 2022 Aug 11:2022.08.11.503614. [Version 1] doi: 10.1101/2022.08.11.503614

Figure 4. nsp10 stimulates hydrolysis without affecting processivity or substrate specificity.

Figure 4.

(A) Effect of nsp10 concentration on the kinetics of nsp14-catalyzed dsRNA hydrolysis. Reactions contained fixed nsp14 (0.1 μM) and varied nsp10 (0 – 2 μM) concentrations. In all cases, nsp14 was pre-mixed with nsp10 on ice 5 min prior to adding to the reaction. Hydrolysis of dsRNA substrate (*P10A-P40:T57) was monitored over a 30 min time course as indicated. Product analysis is shown. (B) Effect of nsp10 concentration on the kinetics of nsp14-catalyzed hydrolysis of ssRNA. Reactions were as in panel A except a ssRNA substrate (*P10A) was used. Product analysis is shown. Products shorter than n-4 are not observed when the 10-nt ssRNA is used.