Transcriptomes and immune responses of mouse corneal cells at 2 dpi and 7 dpi with the P. aeruginosa wild-type PAO1, ΔwspF, and plac-yhjH strains. (A) PCA of the infected mouse corneal cells. Raw RNA-Seq data were normalized using the DESeq package before PCA. (B) Production of cytokines by mouse corneal cells at 2 dpi and 7 dpi with P. aeruginosa ΔwspF and plac-yhjH strains. Black and gray bars represent 2 dpi and 7 dpi, respectively. Mean values and SD from triplicate experiments are shown. *, P < 0.05, Student's t test.