Table 3.

Output data of central composite design analysis.

Source	VS (nm)		ZP (mV)		EE%
	F	p-value	F	p-value	F	p-value
Model	365.29	<0.0001	201.32	<0.0001	70.86	<0.0001
X1: SPC molar concentration	23.43	0.0005	17.39	0.0011	5.71	0.0342
X2:SDC amount (mg)	57.45	<0.0001	584.86	<0.0001	2.89	0.1149
X3: Cholesterol amount (mg)	1669.75	<0.0001	1.72	0.2127	246.41	<0.0001
X2²	73.31	<0.0001			28.44	0.0002
X3²	33.25	0.0001
Lack of fit	5.63	0.1600	10.52	0.0899	8.46	0.1103
Model	Reduced Quadratic		Linear		Reduced Quadratic
Adjusted R 2	0.9913		0.9741		0.9458
R 2	0.9940		0.9789		0.9594
%CV	1.17		2.51		1.43
Predicted R 2	0.9836		0.9639		0.9189
Adequate precision	58.3942		38.6770		25.5364
SD	0.1552		0.7963		0.0016

$\mathrm{Sqrt}\mathrm{}\left(\mathrm{VS}\right)=13.1\mathrm{}1\mathrm{}-0.237.X_1+0.372.X_2+2.01.X_3+0.763.X_2^2-0.514.$.$X_3^2$

$\mathrm{ZP}=-31.6\mathrm{}6\mathrm{}-1.05.X_1-6.09.X_2-0.33.$.$X_3$

$1/\mathrm{Sqrt}(\mathrm{EE})=0.1\mathrm{}1\mathrm{}-0.001.X_1-0.0009.X_2-0.008.X_3+0.0042$.${.X}_2^2$