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Thermal inactivation curves at 33°C. Infectious virus titres in cell culture wells at 33˚C (A). The horizontal dotted line denotes the limit of detection in the TCID50 assay. Percentages of infectious viral titre calculated with reference to the titres at 0 h (B). Graphs show the mean values. Error bars indicate SD with N = 3 independent experiments. **P < 0.01; ***P < 0.001; ****P < 0.0001, (Two-way ANOVA followed by Tukey's test).
