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. 1999 Jul;181(13):3886–3889. doi: 10.1128/jb.181.13.3886-3889.1999

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Copyright © 1999, American Society for Microbiology

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FIG. 2 — Cell wall incorporation of HA-tagged proteins. Cell wall fractions were prepared from transformant cells and analyzed by Western blotting. (A) Ypl130w-HA. Lane 1, crude cell lysate; lane 2, cell wall fraction; lane 3, cell wall fraction treated with laminarinase; lane 4, cell wall fraction treated with laminarinase and endoglycosidase H (7). The sample amounts in lanes 2 to 4 were five times more than that in lane 1. (B) Yor382w-HA. Lane 1, crude cell lysate; lane 2, cell wall fraction treated with laminarinase and endoglycosidase H; lane 3, cell wall fraction. The sample amounts in lanes 2 and 3 were five times more than that in lane 1.