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. 2022 Aug 4;61(4):115. doi: 10.3892/ijo.2022.5405

Figure 3.

Figure 3

miR-124-3p delivery through NSC-EXOs is involved in glioma by targeting FLOT2. (A) Venn diagrams illustrating the six potential miR-124-3p targets identified from TargetScan, miRTarBase and miRWalk. (B) Reverse transcription-quantitative PCR analysis of six potential miR-124-3p target genes in glioma cells. (C) FLOT2 protein levels were detected using western blot analysis in glioma cells transfected with control and NSC-EXOs loaded with miR-124-3p mimic or negative control. (D) The potential binding sites of miR-124-3p within the FLOT2 WT and MUT 3'-UTR. Luciferase reporter gene assays were used to analyze the miR-124-3p effect on luciferase activity. *P<0.05, **P<0.01 and ***P<0.001, statistically significant differences between the NC and EXO-miR-124-3p group. NC, miR negative control; NSC, neural stem cell; EXOs, exosomes; FLOT2, flotillin 2; siFLOT2, siRNA FLOT2; WT, wild-type; MUT, mutant.