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. 2022 May 19;100(8):skac189. doi: 10.1093/jas/skac189

Table 5.

Effect of dietary supplementation of exogenous alpha-amylases (AMA) on rumen pH, volatile fatty acids, and ammonia nitrogen concentrations by lactating dairy cows

Item Control2 RMD3 Variance component4 Bias
N1 Mean STD Effect size P 2 τ2 Funnel Test5
(P-value)
I 2 (%)
pH 12 6.3 0.3 −0.03 (−0.06,0.01) 0.10 0 0 0.1 0
Total VFA6 (mmol/L) 16 111.9 45.6 0.86 (−4.56, 6.3) 0.66 9.06 0 0.07 73.48
Acetate (%) 17 62.5 3.3 −0.32 (−1.23,0.59) 0.38 2.18 0 0.62 42.90
Propionate (%) 17 20.0 2.6 0.03 (−1.41,1.46) 0.95 0.64 0 0.91 60.52
Butyrate (%) 17 13.4 1.9 0.14 (−0.65,0.93) 0.55 0.27 0 0.73 43.48
A:P ratio7 13 3.10 0.5 −0.10(−0.42,0.23) 0.41 0.15 0 0.38 73.59
NH3−N (mg/dL) 16 21.0 6.5 0.06(−0.78,0.91) 0.84 0 0 <0.01 71.63

Positive values in RMD indicate an increase by addition of AMA, whereas negative values in RMD indicate a decrease by addition of AMA respect to the control.

Total number of comparisons.

No AMA in the diet.

Raw mean difference (weighted) between control vs. AMA treatment.

2 = between-studies-within-cluster variance component, τ2 = between-cluster variance component (Hedges et al., 2010; Fisher et al., 2017).

P-value for X2 (Q) test for heterogeneity; I2 = Proportion of total variation of size effect estimated due to heterogeneity.

Total volatile fatty acids.

Acetate:Propionate ratio.