Figure 1.

HDL anti-inflammatory effect and ability to induce cholesterol efflux. (A) Anti-inflammatory effect was measured on HUVEC incubated with HDL (800 µg/mL apoAI) for 16 hours before stimulating VCAM-1 and ICAM-1 expression by 100 UI/mL recombinant TNFα. HDL-induced inhibition was calculated by comparison with TNFα-induced VCAM-1 and ICAM-1 expression without HDL. (B) Cholesterol efflux was measured on THP-1 macrophages loaded with Bodipy-labeled cholesterol and incubated with apolipoprotein B-depleted plasma (5% v/v) for 4 hours. Percent of efflux was calculated with the following formula: [fluorescence in supernatant ÷ (fluorescence in supernatant + fluorescence in lysat)] × 100. NS, not significant.