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. Author manuscript; available in PMC: 2023 Feb 25.
Published in final edited form as: Sci Total Environ. 2021 Oct 27;809:151118. doi: 10.1016/j.scitotenv.2021.151118

Fig. 1. Nrf2 was upregulated in Cr-T cells.

Fig. 1.

(A) BEAS-2B cells were treated with 1 µM of Cr(VI) for 6 months. These Cr-T cells were transformed and formed colonies in vitro and tumor in vivo. Single colonies were isolated from Cr-T cells above. Passage matched parental BEAS-2B (B2B) cells were used as control. The expression levels of Nrf2 and β-actin were determined using immunoblotting assay in 7 different Cr-T colonies. (B) Nrf2 and β-actin expression in pooled Cr-T cells. (C) The expression of Nrf2 in cytoplasm and nuclear fraction. α-tubulin and Histone 3 were used as an internal control for cytoplasm and nuclear fraction, respectively. (D) Immunofluorescence staining for Nrf2 (red) and DAPI (blue). Magnification: 400x. (E and F) BEAS-2B cells were treated with 1 µM of Cr(VI) for 1, 2 and 3 months. The mRNA levels of NQO1 and HO-1 were measured using real-time PCR. **, p < 0.01 compared to parental B2B cells. ***, p < 0.001 compared to parental B2B cells.