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. Author manuscript; available in PMC: 2023 Feb 25.
Published in final edited form as: Sci Total Environ. 2021 Oct 27;809:151118. doi: 10.1016/j.scitotenv.2021.151118

Fig 8. Forced expression of Nrf2 restored miR-27a/b-inhibited cell proliferation, colony formation, migration and tube formation.

Fig 8.

Fig 8.

Cr-T cells were transient transfected with miR-NC, miR-27a, miR-27b with vector control or Nrf2 ORF plasmid. (A) The expression of Nrf2 was analyzed 72 h after transfection using immunoblotting assay. After 24 h post transfection, cells were trypsinized and seed for cell proliferation assay using MTT method (B), colony formation assay using soft agar (C), transwell migration assay (D), and tube formation assay (E). Quantitative results were shown using bar graph from three independent experiments. * and #, p < 0.05 compared to miR-NC group and miR-27a/b + Nrf2 group, respectively. ** and ##, p < 0.01 compared to miR-NC group and miR-27a/b + Nrf2 group, respectively.