Skip to main content
. Author manuscript; available in PMC: 2023 Jul 15.
Published in final edited form as: J Immunol. 2022 Jun 27;209(2):368–378. doi: 10.4049/jimmunol.2101172

FIGURE 4.

FIGURE 4.

LRP5/6 signaling in macrophages regulates the expression of proinflammatory cytokines and activation of MAPKs. (A, B) Quantitative real-time PCR analysis of the expression of Wnt ligands in splenic and kidney macrophages (Mϕs) isolated from LRP5/6Flox mice treated with or without DSS on day 8. (C, D) Quantitative real-time PCR analysis of the mRNA of indicated genes in splenic and kidney macrophages from DSS-treated LRP5/6Flox and LRP5/6LysM mice (day 8). (E) Sorted splenic macrophages from untreated and DSS-treated LRP5/6Flox and LRP5/6LysM mice were cultured for 2 days ex vivo and cytokine levels in the culture supernatants were quantified by ELISA. (F) Representative histogram of phosphorylated p38, JNK, and ERK MAPKs in splenic macrophages isolated from untreated and DSS-treated LRP5/6Flox and LRP5/6LysM mice (day 8). Data is representative of two experiments (n = 4–5 mice per experiment). Error bars show mean values ± SEM. *p<0.05; **p<0.01; ***p<0.001.