TABLE 1.
Kinetic and equilibrium constants of AcPhe-puromycin synthesis carried out in the presence of spermine analogues, with complex C formed in the absence of the FWR fraction
| Effectora | k3b (min−1) | Ksb (μM) | Kic (μM) | βc |
|---|---|---|---|---|
| None (control) | 1.11 | 660 | ||
| SPM | 118 | 0.165 | ||
| N1-MESPM | 138 | 0.138 | ||
| N1,N12-BESPM | 225 | 0.360 | ||
| N4,N9-BESPM | 222 | 0.345 | ||
| N4,N9-DBzSPM | 130 | 0.522 |
a
SPM, spermine; N1-MESPM, N1-ethylspermine; N1,N12-BESPM, N1,N12-bis(ethyl)spermine; N4,N9-BESPM, N4,N9-bis(ethyl)spermine; N4,N9-DBzSPM, N4,N9-dibenzylspermine.
b
The k3 and Ks values were calculated by fitting the experimental data to equation 2 by a least-square procedure provided by Microcal Software, Inc.
c
The Ki and β values were obtained from the corresponding 1/Δ intercept replots.