FIG. 2.

Cellular localization of PelW in E. coli. The proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the gels were autoradiographed. E. coli BL21(DE3)/pT7-KC (PelW), E. coli BL21(DE3)/pT7-6 (pT7-6), E. coli BL21(DE3)/pT7-KCsp (PelW-SP), and E. coli K38/pGP1.2/pT-pelD (PelD) cells were fractionated and then labelled with [³⁵S]cysteine-[³⁵S]methionine in the absence (−) or in the presence (+) of CCCP. W, whole-cell lysate; P, periplasmic fraction; C, osmotically shocked cell fraction. Precursor (p) and mature (m) forms of PelW-SP and PelD are indicated by arrowheads.