TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Bacterial strains | ||
| E. chrysanthemi 3937 derivatives | ||
| A350 | lacZ2 | 5 |
| A1077 | lacZ2 kdgR::Cm | Laboratory collection |
| A1671 | lacZ2 pelW::MudI1734 Km | 5 |
| A3439 | lacZ2 kdgR::Cm pelW::MudI1734 Km | This work |
| Escherichia coli | ||
| NM522 | Δ(lac-proAB) Δ(mcrB-hsdSM)5 supE thi (F′ proAB lacIqlacZΔM15) | Laboratory collection |
| BL21(DE3) | E. coli B; F−dcm ompT hsdS gal λ(DE3); T7 polymerase gene under the control of the lacUV5 promoter | Stratagene |
| K-38 | HfrC(λ) phoA4 pit-10 tonA22 ompF627 relA1 | Laboratory collection |
| Plasmids | ||
| pBS Ap | pBluescript SK(+); Apr | Stratagene |
| pET-20b(+) | Carries the N-terminal pelB signal sequence; Apr | Novagen |
| pGP1.2 | The T7 RNA polymerase gene under the control of the λPL promoter and the cI857 repressor; Kmr | 38 |
| pT7-6 | T7Φ10; Apr | 38 |
| pNV9 | pBR325 derivative carrying the 3.2-kb EcoRV-EcoRI fragment with pelW | 5 |
| pBS-KC | pBS derivative with the 2.9-kb EcoRV-ClaI fragment from pNV9; pelW+ | This work |
| pT7-KC | pT7-6 derivative with the 3-kb EcoRV-EcoRI fragment from pNV9; pelW+ | This work |
| pT7-KCsp | pET-20b(+) derivative with the 2.5-kb HpaI-HindIII ′pelW fragment under the control of the pelB signal sequence | This work |
| pOGL-10 | pBS derivative carrying ogl | 28 |
| pT7-OGL | pT7-5 derivative with the 1.6-kb PinAI-PstI fragment from pOGL-10; ogl+ | This work |
| pTaX | pT7-5 derivative with pelX; Apr | 34 |
| pT7-pelD | pT7-5 derivative with pelD; Apr | 39 |