Bacterial strains |
E. chrysanthemi 3937 derivatives |
A350 |
lacZ2 |
5 |
A1077 |
lacZ2 kdgR::Cm |
Laboratory collection |
A1671 |
lacZ2 pelW::MudI1734 Km |
5 |
A3439 |
lacZ2 kdgR::Cm pelW::MudI1734 Km |
This work |
Escherichia coli
|
NM522 |
Δ(lac-proAB) Δ(mcrB-hsdSM)5 supE thi (F′ proAB lacIqlacZΔM15) |
Laboratory collection |
BL21(DE3) |
E. coli B; F−dcm ompT hsdS gal λ(DE3); T7 polymerase gene under the control of the lacUV5 promoter |
Stratagene |
K-38 |
HfrC(λ) phoA4 pit-10 tonA22 ompF627 relA1
|
Laboratory collection |
Plasmids |
pBS Ap |
pBluescript SK(+); Apr
|
Stratagene |
pET-20b(+) |
Carries the N-terminal pelB signal sequence; Apr
|
Novagen |
pGP1.2 |
The T7 RNA polymerase gene under the control of the λPL promoter and the cI857 repressor; Kmr
|
38 |
pT7-6 |
T7Φ10; Apr
|
38 |
pNV9 |
pBR325 derivative carrying the 3.2-kb EcoRV-EcoRI fragment with pelW
|
5 |
pBS-KC |
pBS derivative with the 2.9-kb EcoRV-ClaI fragment from pNV9; pelW+
|
This work |
pT7-KC |
pT7-6 derivative with the 3-kb EcoRV-EcoRI fragment from pNV9; pelW+
|
This work |
pT7-KCsp |
pET-20b(+) derivative with the 2.5-kb HpaI-HindIII ′pelW fragment under the control of the pelB signal sequence |
This work |
pOGL-10 |
pBS derivative carrying ogl
|
28 |
pT7-OGL |
pT7-5 derivative with the 1.6-kb PinAI-PstI fragment from pOGL-10; ogl+
|
This work |
pTaX |
pT7-5 derivative with pelX; Apr
|
34 |
pT7-pelD
|
pT7-5 derivative with pelD; Apr
|
39 |