TABLE 1.
L. casei strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| L. casei strains | ||
| BL23 | ATCC 393[pLZ15−] | B. Chassy (University Illinois, Urbana) |
| BL23D | man | 49 |
| BL71 | ccpA | 34 |
| BL72 | man ccpA | 22 |
| BL153 | BL23 lacE | This work |
| BL154 | BL23 lacT | This work |
| BL155 | BL23 lacF | This work |
| Plasmids | ||
| pUC18 | Amr | Boehringer Mannheim |
| pRV300 | Ermr from pAMβ1 | 30 |
| pBluescriptII SK+ | Amr | Stratagene |
| pT7Blue-T-vector | Amr | Novagen |
| pNZ272 | gusA Cmr | 36 |
| pNZlac | pNZ272 containing the cre element of lacp fused to gusA | 34 |
| pNZRAT | pNZ272 containing the cre element and RAT-terminator of lacp fused to gusA | This work |
| pMJ39 | lacE with 0.965-kb deletion in pRV300 | This work |
| pMJ41 | lacT with a frameshift at PstI site in pRV300 | This work |
| pMJ45 | lacF with a frameshift at SphI site in pRV300 | This work |
| pMJ33 | pBluescript containing a fragment of lacp (Fig. 1) | This work |
| pMJ64 | pBluescript containing a fragment of lacE and lacG (Fig. 1) | This work |