Fig. 3.

In vivo Mecp2-targeted RNA editing leads to expression of AAV-encoded Editase^wt and rescues endogenous MeCP2 protein expression. (A) Confocal images of sections of brainstem and a portion of cerebellum stained for Editase^wt and MeCP2 protein. DAPI staining indicates nuclei. (Upper) Mecp2^G311A/y mice injected with nontargeting gfp guide virus. (Lower) Mecp2^G311A/y mice injected with Mecp2-targeting virus. Editase^wt is expressed in both conditions while MeCP2 immunolabeled nuclei are detected only in the mice injected with Mecp2-targeting virus. (Scale bar, 500 µm.) (B, Upper) Percent Editase immunopositive cells in both viral conditions. (Lower) Percent MeCP2 immunopositive cells in both conditions. Data are relative to total number of DAPI⁺ cells. n = 3 mice each condition.