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. 2022 Aug 8;119(33):e2117903119. doi: 10.1073/pnas.2117903119

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Copyright © 2022 the Author(s). Published by PNAS.

This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 2. — D₁-receptor–dependent protein phosphorylation by noradrenaline in the dentate gyrus. (A) Effects of NA on GluA1 phosphorylation in dentate gyrus (DG) (n = 7 for each point) and striatum (n = 4). Typical immunoblots for detection of phospho-Ser845 (left) and quantified data (right). One-sample t test (**P = 0.0019, ****P < 0.0001, compared with 1). (B) Effects of SKF83566 (500 nM) and propranolol on NA-induced GluA1 phosphorylation. Typical immunoblots (top) and quantified data (bottom). Two-way ANOVA (SKF effect, F_1,32 = 19.28, P = 0.0001; Pro effect, F_1,32 = 22.07, P < 0.0001) followed by Tukey's test (**P < 0.005, ****P < 0.0001). (C) Effects of noradrenaline on DARPP32 phosphorylation (phospho-Thr34) in slices of DG and striatum. One-sample t test (**P < 0.005). DG: n = 6, striatum: n = 4. (D) Antagonist effects on NA-induced phosphorylation of DARPP32 in DG. Two-way ANOVA (SKF effect, F_1,32 = 8.549, P = 0.0063; Pro effect, F_1,32 = 9.497, P = 0.0042) followed by Tukey's test (***P = 0.0006). (E) Effects of noradrenaline on ERK2 phosphorylation in slices of DG and striatum. One-sample t test (*P < 0.05, **P < 0.01). DG: n = 7, striatum: n = 4. (F) Antagonist effects on NA-induced phosphorylation of ERK2 in DG. Two-way ANOVA (SKF effect, F_1,32 = 11.65, P = 0.0018) followed by Tukey's test (*P = 0.0123, **P = 0.0025). Phospho-proteins were normalized to total proteins and then data were normalized to values obtained with time 0 (A, C, and E) or control without noradrenaline (B, D, and F).