Fig. 4.

Interaction between D₁ and β receptors. (A) Schematic diagram showing experimental design of coimmunoprecipitation assay using tagged receptors. IP: immunoprecipitation, IB: immunoblot. (B) Coimmunoprecipitation of FLAG-tagged D₁ and HA-tagged β₁ or β₂ receptors expressed in HEK293T cells. (C) NA-induced cAMP production in HEK293T cells expressing EGFP or D₁ receptors (n = 2 each). (D) Experimental design of coimmunoprecipitation assay of native receptors. (E) Coimmunoprecipitation of hippocampal D₁ and β₁ receptors in control and mice treated with 11 times of ECT (ECTx11). IgG: immunoglobulin G. Experiments were repeated three times with similar results (SI Appendix, Fig. S5B).