FIG. 1.

Diagrammatic representation of hybrid and wild-type RubisCO holoenzyme expression plasmids. The plasmids were named such that the origin of each subunit gene is indicated by the subscripted first letter of the species from which it originated. The bold arrow indicates the location of the lac promoter with respect to the cbb genes, which are all in the proper transcriptional orientation for controlled expression with this promoter. Open and shaded rectangles represent coding regions originating from R. sphaeroides and R. capsulatus, respectively. Abbreviations for restriction sites: H, HindIII; X, XbaI; B, BamHI; and K, KpnI.