TABLE 3.
Activity of R. sphaeroides-R. capsulatus hybrid and chimeric recombinant RubisCO in extracts of E. coli
| E. coli plasmida | Source of RubisCO (genes or protein) | Activityb (μmol/min) |
|---|---|---|
| pLsSs | R. sphaeroides cbbL-cbbS | 20.4 |
| pLsSc | R. sphaeroides cbbL-R. capsulatus cbbS | 0.00 |
| pLsSc IBFc | R. sphaeroides cbbL-R. capsulatus cbbS | 0.00 |
| pLsSc IBF + pBGL520d | R. sphaeroides cbbL-R. capsulatus cbbS + Synechococcus strain PCC 6301 rbcL | 1.20 |
| pLsSc + cyanobacterial small subunitse | R. sphaeroides cbbL-R. capsulatus cbbS + purified Synechococcus sp. strain PCC 6301 RbcS | 0.00 |
| ps/cL-XhoI IBF | Chimeric R. sphaeroides-R. capsulatus cbbL/R. capsulatus cbbS | 0.00 |
| ps/cL-XhoI IBF + pBGL520d | Chimeric R. sphaeroides-R. capsulatus cbbL/R. capsulatus cbbS + Synechococcus rbcL | 1.80 |
| ps/cL-XhoI + purified cyanobacterial small subunits | Chimeric R. sphaeroides-R. capsulatus cbbL/R. capsulatus cbbS + purified Synechococcus RbcS | 0.00 |
| pBGL520 | Synechococcus strain PCC 6301 rbcL | 0.10 |
| Purified cyanobacterial small subunits | Purified Synechococcus strain 6301 RbcS | 0.00 |
| pBGL520 + purified cyanobacterial small subunits | Synechococcus strain PCC 6301 rbcL and purified RbcS | 1.30 |
a
Extracts were obtained from E. coli JM109 containing the indicated plasmids (Fig. 1).
b
Represents either total RubisCO or reconstitution activity obtained from 1 liter of cells. Reconstitution assays were performed with saturating concentrations of cyanobacterial large and small subunits where indicated.
c
IBF, 4 M urea-solubilized inclusion body fraction.
d
pBGL520 represents cell extract from E. coli cells expressing Synechococcus strain PCC 6301 large subunits only.
e
Purified from extracts of E. coli(pBLG535) expressing Synechococcus strain PCC 6301 small subunits only (12).