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Influence of ςB on survival of freezing and thawing. One hour after cultures of the wild-type strain (squares) and the sigB mutant (triangles) had entered the stationary phase as a result of the exhaustion of glucose, samples were removed and immediately frozen at −20°C. Each day, aliquots were thawed at room temperature and appropriate dilutions were plated on LB agar plates. The number of cells present during the sampling was set at 100%.
