Figure 2.

(A–C) The T-shaped channel design (A) was first created in 100 μm-thick SU-8 resin (B and C) then negatives were replicated in PDMS. (D and E) The supported bilayer was formed on the right-hand side of the channel by injecting both large unilamellar vesicles and buffer (D), followed by closing ports 2 and 3 and using flow to drive the supported bilayer toward port 4 (E). The leading edge of the bilayer was imaged while moving between the location indicated by arrow (c) toward arrows (b) and (a). To see this figure in color, go online.