TABLE 2.
Muropeptide identities and quantification from peptidoglycan of B. subtilis HR, AA106 (ponA), and AA109 (dacA)
| Muro-peptidea | Identity | Mol%
|
||
|---|---|---|---|---|
| HR (wild type) | AA106 (ponA) | AA 109 (dacA) | ||
| 1 | Disaccharide tripeptide | 3.3 | 2.9 | 0.7 |
| 2 | Disaccharide tripeptide with 1 amidation and 1 phosphate | 0.9 | 1 | |
| 3 | Disaccharide tripeptide with 1 amidation | 20.5 | 28.9 | 1.3 |
| 4 | Disaccharide tripeptide 1 amidation missing an acetyl group | 3.4 | 4.3 | 0.3 |
| 5 | Disaccharide dipeptide | 3.8 | 4.4 | 3.7 |
| 6 | Disaccharide tripeptide dipeptide with 1 amidation | 0.3 | 0.7 | |
| 7 | Disaccharide tetrapeptide with 1 amidation | 0.5 | 0.9 | 0.5 |
| 8 | Disaccharide tripeptide dipeptide with 2 amidations | 1.6 | 0.3 | |
| 9 | Disaccharide pentapeptide (Ala-4–Gly-5) with 1 amidation | 0.5 | 1.6 | 1.9 |
| 10 | Disaccharide tripeptide tetrapeptide with 2 amidations | 0.5 | 0.2 | 0.4 |
| 11 | Disaccharide pentapeptide (Ala-4–Ala-5) with 1 amidation | 0.6 | 2.1 | 19.1 |
| 12b | Disaccharide tripeptide disaccharide tetrapeptide missing a glucosamine | 0.4 | 1.1 | |
| 13 | Disaccharide tripeptide disaccharide tetrapeptide with 1 phosphate and 1 amidation | 0.5 | 0.3 | |
| 14 | Disaccharide tetrapeptide tetrapeptide with 2 amidations | 0.3 | 0.1 | |
| 15 | Disaccharide tripeptide disaccharide tetrapeptide with 1 amidation | 6.3 | 11.7 | 0.4 |
| 16 | Disaccharide tripeptide disaccharide tetrapeptide with 1 phosphate and 2 amidations | 1.1 | 0.9 | |
| 17a | Disaccharide tripeptide disaccharide tetrapeptide with 1 phosphate and 2 amidations | 0.6 | 0.3 | |
| 17b | Disaccharide tripeptide disaccharide tetrapeptide with 2 amidations and missing a glucosamine | 0.6 | 0.2 | |
| 18 | Disaccharide tripeptide disaccharide tetrapeptide with 1 amidation and missing an acetyl group | 1.2 | 1.9 | |
| 19 | Disaccharide tripeptide disaccharide tetrapeptide with 1 amidation and missing an acetyl group | 0.8 | 1.5 | |
| 20 | Disaccharide tripeptide disaccharide tetrapeptide with 1 amidation | 1.7 | 1.3 | |
| 21 | Disaccharide tripeptide disaccharide tetrapeptide with 2 amidations | 27.7 | 20 | 2.2 |
| 22 | Disaccharide tripeptide disaccharide tetrapeptide with 2 amidations and missing an acetyl group | 5.2 | 2.7 | 0.5 |
| 23 | Disaccharide tripeptide disaccharide tetrapeptide with 2 amidations and missing an acetyl group | 5.0 | 2.9 | 0.8 |
| 24b | Disaccharide pentapeptide (Gly-5) disaccharide tetrapeptide | 1.3 | 0.6 | |
| 25 | Disaccharide pentapeptide (Gly-5) disaccharide tetrapeptide with 2 amidations | 0.8 | 0.4 | 4.1 |
| 26 | Disaccharide tetrapeptide disaccharide tetrapeptide with 2 amidations | 0.4 | 0.4 | 0.5 |
| 27 | Disaccharide pentapeptide disaccharide tetrapeptide with 2 amidations | 1.0 | 2.4 | 30.8 |
| 28 | Disaccharide tripeptide disaccharide tetrapeptide disaccharide tetrapeptide with 2 amidations | 0.5 | 0.4 | |
| 29b | Disaccharide tripeptide disaccharide tetrapeptide disaccharide tetrapeptide missing a glucosamine | 0.5 | 0.1 | |
| 30 | Disaccharide tripeptide disaccharide tetrapeptide disaccharide tetrapeptide with 2 amidations | 1.3 | 1.0 | |
| 31 | Disaccharide tripeptide disaccharide tetrapeptide disaccharide tetrapeptide with 3 amidations | 4.2 | 1.1 | 0.3 |
| 32 | Disaccharide tripeptide disaccharide tetrapeptide disaccharide tetrapeptide with 3 amidations and missing an acetyl group | 1.2 | 0.2 | |
| 33 | Disaccharide tripeptide disaccharide tetrapeptide disaccharide tetrapeptide with 3 amidations and missing an acetyl group | 0.5 | ||
| 34 | Disaccharide pentapeptide (Gly-5) disaccharide tetrapeptide disaccharide tetrapeptide with 2 or 3 amidations | 0.1 | ||
| 35 | Disaccharide tripeptide disaccharide tetrapeptide disaccharide tetrapeptide disaccharide tetrapeptide with 2 or 3 amidations | 0.1 | ||
| 36 | Anhydrodisaccharide tripeptide disaccharide tetrapeptide with 2 amidations | 0.3 | 0.2 | |
| 37 | Disaccharide tripeptide disaccharide tetrapeptide disaccharide tetrapeptide disaccharide tetrapeptide with 4 amidations | 0.2 | ||
| 38 | Anhydrodisaccharide tripeptide disaccharide tetrapeptide disaccharide tetrapeptide with 3 amidations | 0.1 | ||
| A1 | Anhydrodisaccharide tripeptide disaccharide tetrapeptide with 1 amidation | 0.6 | ||
| B1 | Disaccharide pentapeptide | 1.7 | ||
| B2 | Disaccharide pentapeptide missing an acetyl group | 5.0 | ||
| B3 | Disaccharide pentapeptide dipeptide | 1.1 | ||
| B4 | Disaccharide pentapeptide (Gly-5) disaccharide tetrapeptide | 0.3 | ||
| B5 | Disaccharide pentapeptide disaccharide tetrapeptide with 1 phosphate | 0.3 | ||
| B6 | Disaccharide pentapeptide disaccharide tetrapeptide | 1.9 | ||
| B7 | Disaccharide pentapeptide disaccharide tetrapeptide | 0.9 | ||
| B8 | Disaccharide pentapeptide disaccharide tetrapeptide missing an acetyl group | 7.0 | ||
| B9 | Disaccharide pentapeptide disaccharide tetrapeptide missing an acetyl group | 6.8 | ||
| B10 | Disaccharide pentapeptide disaccharide tetrapeptide missing 2 acetyl groups | 2.1 | ||
| B11 | Disaccharide pentapeptide disaccharide tetrapeptide disaccharide tetrapeptide | 0.3 | ||
| B12 | Disaccharide pentapeptide disaccharide tetrapeptide disaccharide tetrapeptide | 0.4 | ||
| B13 | Disaccharide pentapeptide disaccharide tetrapeptide disaccharide tetrapeptide | 1.8 | ||
| B14 | Disaccharide pentapeptide disaccharide tetrapeptide disaccharide tetrapeptide missing an acetyl group | 1.1 | ||
| B15 | Disaccharide pentapeptide disaccharide tetrapeptide disaccharide tetrapeptide missing an acetyl group | 0.6 | ||
| B16 | Disaccharide pentapeptide disaccharide tetrapeptide disaccharide tetrapeptide disaccharide tetrapeptide | 0.2 | ||
| B17 | Anhydrodisaccharide pentapeptide disaccharide tetrapeptide | 0.7 | ||
a
Numbered as indicated in Fig. 1.
b
Muropeptide from B. subtilis HR (wild type) not analyzed for amide levels due to lack of material.